Hybrid onsite (3 days/week)
Lab-based position, will help process PBMCs and FFPE tissues for scRNAseq and fixed RNA profiling workflows, scRNAseq library preparation and QC.
PhD recent grad will be considered.
* Industry experience preferred with molecular biology techniques.
* Bachelors with 10 years experience will be overqualified.
* Masters with 1 year experience (academic or industry) preferred.
* Interpret data.
* Lab-based role.
* Molecular biology experience.
* Use a lot of automation to process samples.
* scRNAseq experience.
* Sample processing workflows using automation.
* Majority of lab work.
* Bachelors with 3-5 years experience.
* Masters degree with over 5 years experience will be overqualified.
* Quality control of NGS Libraries (not a must have).
Top skills:
* Nucleic isolation background.
* Handling cells PBMC and tissues.
* Tissue disassociation.
* Liquid handling experience (Not a must have, Biomek).
Top 3-5 skills, experience or education required for this position:
* Experience with scRNAseq workflows (10x Genomics preferred) including library preparation and QC is required.
* Experience handling PBMCs, primary cells, and/or tissues is required.
* Background in biology, immunology, molecular biology, or other related field.
* Experience with automated liquid handlers is preferred but not required.
* Experience working with large sample cohorts/in a team environment is preferred.
Key Responsibilities:
* Execute high-throughput scRNAseq library preparations by working effectively on a team and leveraging the use of automated liquid handlers (e.g., Biomek i7).
* Operate Illumina sequencers and other lab instrumentation for quality control of NGS Libraries (e.g., Tapestation).
* Accurately document project and sample information, protocols, and experimental details through the Electronic Lab Notebook (ELN).
* Conduct experiments, troubleshoot, and optimize protocols independently and in collaboration with colleagues to deliver high-quality data in a timely fashion.
* Interpret findings and communicate effectively to present scientific data, concepts, and recommendations to various internal audiences.
* Interface with computational biologists and data scientists to generate and interpret scRNAseq datasets.
* Work in client’s matrixed environment and support activities for different groups across the organization, which may include alignment meetings.
Qualifications:
* BS or equivalent education with 3+ years of experience or MS or equivalent education with typically 2+ years of experience.
* Strong background in basic molecular biology and/or genomics with proficiency in molecular biology lab techniques such as DNA/RNA extraction and nucleic acid QC, as well as familiarity with PBMC isolation and handling.
Preferred Qualifications:
* Experience with nuclei isolation from PBMCs and/or tissues is preferred.
* Familiarity with 10X Genomics platforms, particularly scRNAseq, Fixed RNA profiling, and/or scATACseq, is preferred.
* Familiarity with automated liquid handlers such as Biomek i7 or Tecan Fluent is a plus but not required.
* Background in R is a plus but not required.
* Background in immunology is a plus but not required.
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