About the role
To assist the existing investigators by performing laboratory-based research to understand the immune response, and to isolate and characterise antibodies from patients with systemic amyloidosis. Experimental techniques will include multiparameter flow cytometry for phenotypic characterisation and isolation of memory B-cells, population and single cell BCR sequencing and cloning, recombinant protein production, antibody purification, and assays of antibody binding characteristics, including ELISA and in vitro cell culture assays.Duties and responsibilities Include the following:Perform experiments; maintain an accurate lab book and file their data in a form accessible to other members of the lab; maintain and update laboratory standard operating procedures and risk assessments as required; maintain accurate and up-to-date liquid nitrogen/freezer storage and database recording for samples; order and maintain lab stocks, effective running of laboratory equipment, trouble-shooting problems where necessary; carry out any other duties as are within the scope, spirit and purpose of the job as requested by the PIs. The nature of scientific work, and in particular work with patient samples, means it may be necessary to work outside normal working hours in order to process tissue samples and complete experiments. Note: this job description reflects the present requirements of the post. As duties and responsibilities change and develop the job description will be reviewed and be subject to amendment in consultation with the post holder.
About you
Key requirements include BSc, MSc and/or PhD for appointment at Grade 7 in Biology / Immunology / Biomedical Sciences or related subject General laboratory skills Experience of working in a research laboratory Experience in human cellular immunology Good level of competency at mammalian cell culture Good level of competency at multiparameter flow cytometry Experience of molecular biology (PCR/DNA purification, cloning) Evidence of outstanding attention to detail and a methodical approach Accurate and complete record keeping and ability to manage time and work to strict deadlines.
What we offer
This is an exciting opportunity to join a multidisciplinary team working across the Division of Medicine. As well as the exciting opportunities this role presents, we also offer some great benefits some of which are below: • 41 Days holiday (27 days annual leave 8 bank holiday and 6 closure days) • Additional 5 days’ annual leave purchase scheme • Defined benefit career average revalued earnings pension scheme (CARE) • Cycle to work scheme and season ticket loan • Immigration loan • Relocation scheme for certain posts • On-Site nursery • On-site gym • Enhanced maternity, paternity and adoption pay • Employee assistance programme: Staff Support Service • Discounted medical insurance