I need a freelancer who is expert in writing original article by extracting data from the following portals. 1.The NCBI GEO database (http://www.ncbi.nlm.nih.gov/geo) 2. The original SRA file should be downloaded and processed using SRA-Toolkit (https://github.com/ncbi/sra-tools/wiki/01.-Down loading-SRA-Toolkit) and Salmon (https://salmon.readthedocs.io/en /latest/salmon.html) to generate FASTQ and gene count files for further analysis. 3.Additionally, the GSE235995 expression profiling dataset,should be sequenced using the Illumina NovaSeq 6000 (GPL24676) for Homo sapiens, was acquired from the GEO database. 4. This dataset which will be divided in to 2 groups should include five calcific aortic valve disease samples and four normal control samples. 5. Data standardization and log₂ (data1) transformation should be performed using the Python and R package ‘DESeq2’ [10] (https://biocon ductor.org/packages/release/bioc/html/DESeq2.html), and this dataset will be used for validation.