Job Responsibilities
* Grow and maintain primary, mammalian and specialised cell lines (suspension, adherent, 3D organoids) to be used in biological assay work.
* Maintain cell biobank of patient derived cell lines.
* Prepare conditioned media for successful maintenance of cells.
* Working with complex cell culture models (ALI Cultures)
* Cell fixing, staining and imaging
* Experience with any of the following is preferable: Epithelial Cell Culture, Western Blot, isolation, Flow Cytometry (including analysis of data), ELISA or MSD assays
* Ensure aseptic technique is adhered to in the maintenance of cells
* Aherence to Health and Safety procedures for own well-being and quality of product.
* Actively seek opportunities to develop the service by discussion with customer or by the sharing of best practice with other groups.
* Assist with the preparation of SOPs, Risk Assessments and best practice guides.
* Act as an ambassador for the Technical Laboratory Services Group and promote PerkinElmer’s core values.
* Operate to approved quality standards.
* Work independently and flexibly depending on team priorities and set realistic work schedules to support requirements.
* Perform any other reasonable duties as requested by the line manager or project team leader.
Preferred Education/Experience/Qualifications
* BSc or equivalent in relevant scientific subject (e.g. biology, biotechnology or Biomedical Sciences), with relevant laboratory experience.
* 1 year of demonstrated technical expertise/or equivalent in cell culture, general lab instrumentation, cell-based assays, immunoassays such as ELISA – This can be through an academic experience or workplacement
* Demonstrateable knowledge or understanding of cell culture, cell lines and aseptic technique.
* Understanding of standard software packages (i.e.: Microsoft Office)
* Highly motivated and independent, yet able to work well in a group setting.
* Ability to work independently and deliver high-quality, consistent results in a timely fashion.
* Ability to meet timelines successfully with the utmost attention to detail while setting high performance standards.
* Ability to understand, interpret, and communicate scientific data.
* Ability to execute new and established methods and protocols.
* Outstanding communication and interpersonal skills
Physical Demands
The overall physical exertion of this position requires:
* Sit or stand 4-5 hours in laboratory areas.
* Work around moving laboratory equipment and machinery.
* Work with biohazards such as blood borne pathogens.
* Hepatitis B vaccination will be required, Vaccination programme in place for successful candidates who are not already vaccinated or require updated boosters.
* All work is carried out in a containment level 2 environment.
* Appropriate PPE must always be worn (Lab coat, gloves, eye protection).
* Reach above/below the shoulder, bend at the knees and waist.
* Office work activities, including keyboard, up to 2-3 hours.
Working Environment
* Must be able to work in a laboratory, controlled environments requiring personal protective equipment.
* Job pace may be fast and job completion demands may be high.
* Employee maybe required to manage hazardous wastes in compliance with company procedures & State/Federal/Local hazardous waste regulations. Duties may include: Identifying, handling, generating, accumulating, storing, labelling.
* Ensure safety, security, and the environment in all aspect of the daily activities, and any potential safety hazards are addressed and corrected immediately.
* Understand ergonomic relationship between people, equipment and working environment.
#J-18808-Ljbffr