Job Description The role of Scientist I/II (Upstream) is to support TNAC’s R&D function by following SOPs, as directed by senior staff in areas such as molecular biology, cell culture, and protein purification. The role will be mainly lab-based. Key roles and responsibilities include, but are not limited to: Working proficiently and independently to existing SOPs in the following fields: Molecular Biology. Construction of expression vectors using standard techniques such as: RNA and DNA extraction; preparative and analytical PCR; reverse-transcription PCR; restriction digestion; agarose electrophoresis; Gibson assembly; preparation of transfection-grade plasmids at various scales; Sanger and Nanopore sequencing. Bacterial cell culture. Preparation of growth media; transformation of bacteria; expression testing and optimisation of culture conditions; native and recombinant protein expression at various scales; harvesting and QC testing of raw materials. Mammalian cell culture. Maintenance of cell lines; performing small- and large-scale transfections; harvesting and QC testing of raw materials. Protein purification and analysis. Preparation of suitable buffers; small-scale FPLC purification of target proteins using spin columns; SDS-PAGE; Western blotting; and ELISA. Day-to-day reporting of results to line manager(s), as required. Helping with ordering and maintaining stock levels of high-turnover items and reagents. Meticulous record-keeping. Weekly supervisor meetings. Attendance and presentation at weekly lab meetings. Developing an ability to understand and convey relevant published research. Additional duties, as required by senior R&D staff. The Scientist I/II (Upstream) will also be able to demonstrate the following traits in line with LGC’s Core Values: Passion: Pride in our achievements engenders passion for our work and translates into our shared ambition to make the world a safer place. Curiosity : We constantly look forward to what's next: our curiosity for today's discoveries makes tomorrow's innovations possible. Integrity : A commitment to high ethical standards has been at the heart of LGC since we first started testing the integrity of products in 1842. Brilliance: We strive to be the very best in everything we say and do. We exceed customers' expectations through innovation and pioneering science. Respect: We respect gender, age, nationality, religion, and individuality - our diversity is our strength.