Nucleome Therapeutics, is using breakthrough genomics technologies to discover first-in-class antibody therapeutics for inflammatory diseases. The approach is based solely on human disease genetics and biology. Nucleome is able, for the first time, to reveal molecular mechanisms of disease and select drug targets using human genetic variants in the non-coding genome that alter gene expression. The Company’s proprietary 3D genomics combines lab and machine learning technologies which can be applied to any set of disease-associated variants. Nucleome Therapeutics was founded by leading experts in gene regulation from the University of Oxford and is backed by top tier life science investors. Overview of the role: The drug discovery team is looking for a talented Senior Scientist with a background in antibody characterisation and analysis using primary immune cells to support the pipeline of drug targets emerging from Nucleome’s proprietary platform technology. In this lab-based role you will design, develop and prosecute assays for antibody screening and evaluation, target validation, and mechanism of action studies to support the progression of multiple asset programmes in early drug discovery. You will work with a team of experienced drug discovery scientists and have the opportunity to broaden your expertise and understanding of drug discovery. In your position you will work in close collaboration with other scientists across cross-functional teams and will have responsibility for delivery of defined studies. You will achieve visibility for your work by frequent presentation across team meetings and collaborative interactions. Key responsibilities: Perform research activities to support the progression of multiple asset programmes in early drug discovery with direction from project/team lead D esign, optimise and execute assays for evaluation of antibody function in primary immune cells utilising your understanding of the principles of cellular pharmacology related to antibody-based therapeutics (including Fc formats, ADCC/P, Kd, SPR, binding kinetics, antagonism and agonism) Use your knowledge of cellular immunology to design and perform mechanism of action studies to evaluate hypotheses about target biology in primary immune cells Interpret results and suggest next steps with input from project or team lead Deliver high quality, consistent results and ensure data and experimental reports are submitted in a timely manner including use of appropriate software such as GraphPad Prism for statistical analysis and data visualisation Perform multiparameter flow cytometry analysis including designing and analysing complex multicolour panels for surface and/or intracellular proteins, and performing functional assays (e.g. proliferation) Use FlowJo to analyse data appropriately and accurately Troubleshoot and resolve issues with flow cytometers Provide training to others on flow cytometry best practice Advise colleagues on multiparameter flow cytometry analysis including technical considerations for panel design, compensation and gating Collaborate with a team of experienced scientists within and across functions encompassing drug discovery, functional genomics and computational biology Take responsibility for delivery of defined studies or work packages and provide frequent updates on progress Update functional or project lead on progress and highlight risks and propose mitigations Present clear, concise project updates to team (within and across functions) Provide input to decision-making on early-stage targets and drug candidates Comply with relevant policies and procedures for safe use of biological and chemical agents in the laboratory, while also proactively suggesting improvements to workflows EXPERIENCE: MSc or PhD qualification in a relevant field (e.g., immunology), or equivalent industry experience. Extensive experience of assessing antibody function in assays using primary human immune cells. Experience of immune cell phenotyping and functional assays (especially Tregs) Extensive practical experience in conceiving, developing and performing multi-parameter flow cytometry assays for both surface and intracellular markers and functional assays (e.g., proliferation). Experience with ELISA (ideally also multiplex systems e.g., MSD), cytokine profiling and plate-based fluorescence assays. Experience of analysing and visualising data using FlowJo software and performing statistical analyses and data visualisation with GraphPad Prism. SKILLS AND BEHAVIOURS : Strong communication skills and ability to interpret, summarise and communicate results and ideas effectively across multi-disciplinary teams and to company managers. Ability to work independently as well as thrive working in a multidisciplinary team. Willingness to develop and improve new skills and knowledge necessary to successfully support assigned projects. PREFERRED EXPERIENCE: Experience working in drug discovery in an industry setting. Experience in live cell fluorescent imaging (e.g. Incucyte). Experience of transcriptomic or proteomic analysis. Experience with use of R for data analysis and visualisation. Track record of presentations, publications or patents as evidence of research output.