* Grow, maintain and prepare specialised suspension and adherent cell lines (epithelial) to be used in assay work.
* Maintain a frozen cell supply as required.
* Prepare conditioned media for successful maintenance of cells.
* Use of flourescent microscopy for imaging & cell counting
* Supply cells for ad-hoc and routine requests within established timelines.
* Co-ordinate the regular supply of consumables to ensure smooth delivery of cells to biological groups.
* Ensure aseptic technique is adhered to in the maintenance of cells and compliance to Health and Safety procedures for own well-being and quality of final product.
* Actively seek opportunities to develop the service by discussion with customer or by the sharing of best practice with other groups.
* Be an active member of the scientific teams - contribute at team meetings and look for opportunities for innovation.
* Assist with the preparation of SOPs, Risk Assessments and best practice guides.
* To act as an ambassador for TLS and to promote PerkinElmer's core values.
* Operate to approved quality standards.
* Work independently and flexibly depending on team priorities and to set realistic work schedules to support requirements., The overall physical exertion of this position requires:
* Sit or stand for 4-5 hours in laboratory areas.
* Work around moving laboratory equipment and machinery.
* Work with biohazards such as blood borne pathogens.
* All work is carried out in a containment level 2 environment.
* Appropriate PPE must always be worn (Lab coat, gloves, eye protection).
* Reach above/below the shoulder, bend at the knees and waist.
* Office work activities, including keyboard, up to 2-3 hours.
Working Environment
* Must be able to work in a laboratory, controlled environment requiring personal protective equipment.
* Job pace may be fast and job completion demands may be high.
* Employee will be required to manage hazardous waste in compliance with company procedures & State/Federal/Local hazardous waste regulations. Duties may include: identifying, handling, generating, accumulating, storing and labelling.
* Maintain a high standard work environment by ensuring a high level of safety and security is met in all aspects of daily activities, and ensure any potential safety hazards are addressed and corrected immediately.
* Understand ergonomic relationship between people, equipment and working environment.
Good understanding and previous experience of working with epithelial cultures
* Experience in cell differentiation
* Experience in a number of technical analytical procedures, e.g., ELISA, MSD immunoplexes, Flow Cytometry and qPCR
* Data analysis skills (GraphPad, Prism, FlowJo, Excel)
* Experience of working in an industrial environment, BSc in a relevant scientific subject (e.g. biology, biotechnology or Biomedical Sciences), with relevant laboratory experience.
* Demonstrable knowledge of cell culture, cell lines, primary cells and aseptic technique.
* Demonstrable experience of working in a laboratory environment with experience of media preparation, cell isolation techniques and molecular biology methods.
* Understanding of standard software packages (i.e., Microsoft Office).
* Maintain an electronic laboratory notebook and present at team/project meetings.
* Highly motivated and independent, yet able to work well in a group setting.
* Ability to deliver high-quality, consistent results in a timely fashion.
* Ability to meet timelines successfully with the utmost attention to detail while setting high performance standards.
* Ability to understand, interpret, and communicate complex scientific data.
* Experience in design and execution of new and established methods and protocols.